3 Oct Description: pEGFP-N2 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher. Provide pEGFP-N2 vector/plasmid map, full length sequence, antibiotic resistance, size and other information. pEGFP-N2 encodes a red-shifted variant of wild-type GFP which has been optimized for brighter fluorescence and higher expression in mammalian cells.
|Genre:||Health and Food|
|Published (Last):||27 February 2012|
|PDF File Size:||20.37 Mb|
|ePub File Size:||7.91 Mb|
|Price:||Free* [*Free Regsitration Required]|
Create an account to get pegfp n2. Your time is valuable! Vectors for expressing pegfp n2 visualizing a protein of interest fused to AcGFP1, including a prelinearized vector for simple, one-step In-Fusion cloning. All trademarks are the property of Takara Bio Inc.
Sticky ends from different PflFI pegfp n2 may not be compatible. Use with any Living Colors vector that contains a neomycin resistance cassette to create pegfp n2 cell lines. ZsGreen1 has been reported to have better expression than EGFP in lung and muscle tissue and in cultured cells Bell et al.
Vectors for expressing and visualizing a protein of interest fused to AcGFP1, including a pre-linearized vector for simple, one-step In-Fusion cloning.
Certain trademarks may not be registered in all pegfp n2. Our brands Takara Clontech Cellartis. Welcome to the new website for Clontech, Takara and Cellartis products – any questions please contact webmaster takarabio. Prolonged incubation with NdeI may lead to removal of additional nucleotides. As a member of the Takara Bio Group, TBUSA is part of a pegfp n2 that holds a leadership position in the global market and is committed to improving the human condition through biotechnology.
This plasmid has been discontinued by Clontech. Capturem Trypsin Columns may be used to completely digest protein samples in less than pegfp n2 minute with digestion efficiencies protein coverage comparable to or better than those obtained using in-solution trypsin pegfp n2.
A comparative analysis of novel fluorescent proteins as reporters for gene transfer studies. Our pegfp n2 is to develop high-quality innovative tools and services to accelerate discovery.
pegfp n2 There combinant EGFP vector can be transfected into mammalian cells using any standard transfection method. Obtain pegfp n2 tightest possible control of gene expression of your gene of interest and ZsGreen1. Simultaneous, equivalent tet-inducible expression of ZsGreen1 and your protein of interest from the same bidirectional promoter.
An account with takarabio. A bacterial promoter upstream of this cassette expresses kanamycin resistance in E.
Looking for EGFP vectors? Upgrade to AcGFP1 or ZsGreen1 vectors
Efficient cleavage requires at least two copies of the NarI recognition sequence. Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover. Molecular brightness characterization of EGFP in vivo pegfp n2 fluorescence fluctuation spectroscopy.
Deliver and stably express pegfl gene of interest fused to AcGFP1, in a broad range of dividing cells. Oligomerization of green fluorescent protein pegfp n2 the secretory pathway of endocrine cells.
Emerin pEGFP-N2 () | Addgene product information
Measure CRE activity with a ZsGreen1 reporter that has a high signal-to-noise pegfp n2 and a bright signal. Efficient cleavage requires at least two copies of the PluTI recognition sequence. Efficient cleavage pegfp n2 at least two copies of the SfiI recognition sequence.
If required, stable transformants can h2 selected using G 7.
pEGFP-N2载体_质粒图谱 – 优宝生物
Mapping the brain, one cell type at a time Learn pegfp n2 pioneering efforts to map the mammalian brain using single-cell transcriptomics.
EGFP has been pegfp n2 to form dimers Jain et al. Sticky ends from different RsrII sites may not be compatible. Season one Season two Season three BioView blog.
This product utilizes our novel Capturem technology in a pegfp n2 column format with membrane-immobilized trypsin. AcGFP1 is a monomer, which makes it a superior alternative for fusion applications. Sequences flanking Pefp have been converted to a Kozak consensus translation initiation site 6 to further increase the translation efficiency in eukaryotic cells.